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nrStar™ snoRNA PCR芯片(H/M)

  • 产品简介
  • 芯片设计背景
  • 工作流程
  • 数据库
  • 说明书
  •        Arraystar nrStar™ snoRNA PCR Array(H/M)是聚焦核仁小RNA(snoRNA)检测的PCR芯片。nrStar™ Human和Mouse snoRNA PCR芯片分别包含359个和370个权威数据库来源的snoRNA,覆盖了PCR技术可区分的核仁小RNA,是进行snoRNA表达检测与功能研究的有利工具。 


    产品列表

    产品名称 货号 规格 描述
    nrStar™ Human snoRNA PCR Array AS-NR-003-1 384 well / plate 359条snoRNA、7条snoRNA靶向snRNA以及4条snoRNP复合物成员mRNA
    nrStar™ Human snoRNA PCR Array (Roche Light Cycler 480) AS-NR-003-1-R 384 well / plate
    nrStar™ Mouse snoRNA PCR Array AS-NR-003M-1 384 well / plate 370条snoRNAs
    nrStar™ Mouse snoRNA PCR Array (Roche Light Cycler 480) AS-NR-003M-1-R 384 well / plate


    产品特点

    ● 高覆盖度—覆盖PCR技术可区分的snoRNA
    ● 高灵敏度—总RNA要求量可低至50ng,无需扩增
    ● 稳定有效的PCR引物—所有的snoRNA引物均在不同的细胞组织样本中通过严格验证
    ● 简单快速—只需将cDNA与SYBR® Green master mix混合,加入板中,然后运行qPCR,4小时之内就能得到实验结果
    ● 疾病分子标志物筛选—适用于疾病分子标志物的高通量筛选和验证
  •        核仁小RNA (snoRNA)是一类中等长度的非编码小RNA,其长度60-400nt不等,与核仁核糖核蛋白结合形成snoRNPs复合物发挥功能[1]。在脊椎动物中,核仁小RNA的编码基因主要存在于蛋白编码基因的内含子区,经转录加工处理形成成熟的核仁小RNA分子[2]。snoRNA参与多种生物学过程,比如rRNA的加工处理,RNA剪接和翻译,以及氧化应激反应的调控[3]。snoRNA主要通过snoRNPs复合物来发挥作用。通过snoRNA碱基互补识别靶基因特定位点,snoRNPs复合物催化这些位点的2’-O甲基化或假尿嘧啶化反应。根据snoRNA结构与功能的不同,可以将snoRNA分为两大类:负责2’-O甲基化的box C/D snoRNA和负责假尿嘧啶化的box H/ACA snoRNA [1]。另外,还存在一类比较特殊的snoRNA- scaRNAs (它们具有类似box C、box D或者box H、box ACA结构,特异表达于small Cajal body) [4]。snoRNA可以产生更小片段的非编码RNA-sdRNA。sdRNA与miRNA类似,能够通过调节mRNA的稳定性来发挥作用 [5]。

           多项研究表明,snoRNA在肿瘤中异常表达,并在肿瘤的转移过程中扮演着重要角色 [6-7]。在乳腺癌和前列腺癌中,SNORD50A发生了2个碱基的缺失突变,并且其拷贝数明显降低,因此被认为是抑癌基因[8]。有趣的是,H/ACA box snoRNA SNORA42在肺癌中作为癌基因存在,抑制SNORA42的表达具有明显的抗癌作[10]用。C/D box snoRNAs的表达水平常伴随肿瘤的发生而增加 [11]。有一些C/D box snoRNAs可以作为疾病的分子标志物,比如SNORD44和SNORD43与乳腺癌的不良预后相关[9]。snoRNA在神经退行性疾病的发生发展过程也发挥着关键作用。snoRNAs在血液、痰液以及尿液中稳定存在,使其作为疾病分子诊断标志物方面具有巨大的前景。目前,snoRNA已经成为疾病领域特别是癌症领域里的研究热点。
           nrStar™ Human snoRNA PCR芯片包括359条snoRNA、7条snoRNA靶向snRNA以及4条snoRNP复合物成员mRNA,而nrStar™ Mouse snoRNA PCR芯片分别包含370个snoRNA。该PCR芯片覆盖了所有PCR技术可区分的snoRNA,能够快速方便的检测出组织、细胞以及血液中的snoRNA的表达情况。它还可应用于疾病分子标志物的高通量筛选和验证。
           为了确保数据质量,nrStar™ Human snoRNA PCR芯片包含3个针对人snoRNA的内参基因和2个针对人snoRNP复合物成员mRNA的内参基因,而nrStar™ Mouse snoRNA PCR芯片包含8个针对小鼠snoRNA的内参基因用于数据的均一化处理。此芯片还使用RNA Spike-in,PCC和GDC对照组来检测cDNA合成效率,PCR扩增效率和基因组DNA污染。

           为保证nrStar™ snoRNA PCR Array(H/M)的实验效果,建议配套使用Arraystar rtStar™ First-Strand cDNA Synthesis Kit (AS-FS-001)




    参考文献

     1. Esteller M. (2011) "Non-coding RNAs in human disease." Nat. Rev. Genet. 12(12):861-74 [PMID: 22094949] 2. Richard P. and T. Kiss (2006) "Integrating snoRNP assembly with mRNA biogenesis." EMBO Rep. 7(6):590-2 [PMID: 16741502] 3. Williams G.T. and F. Farzaneh (2012) "Are snoRNAs and snoRNA host genes new players in cancer?" Nat. Rev. Cancer 12(2):84-8 [PMID: 22257949] 4. Darzacq X. et al. (2002) "Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs." EMBO J. 21(11):2746-56 [PMID: 12032087] 5. Ender C. et al. (2008) "A human snoRNA with microRNA-like functions." Mol. Cell 32(4):519-28 [PMID: 19026782] 6. Nallar S.C. and D.V. Kalvakolanu (2013) "Regulation of snoRNAs in cancer: close encounters with inter*****." J. Inter***** Cytokine Res. 33(4):189-98 [PMID: 23570385] 7. Mannoor K. et al. (2012) "Small nucleolar RNAs in cancer." Biochim. Biophys. Acta 1826(1):121-8 [PMID: 22498252] 8. Siprashvili Z. et al. (2016) "The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer." Nat. Genet. 48(1):53-8 [PMID: 26595770] 9. Gee H.E. et al. (2011) "The small-nucleolar RNAs commonly used for microRNA normalisation correlate with tumour pathology and prognosis." Br. J. Cancer 104(7):1168-77 [PMID: 21407217] 10. Mei Y.P. et al. (2012) "Small nucleolar RNA 42 acts as an oncogene in lung tumorigenesis." Oncogene 31(22):2794-804 [PMID: 21986946] 11. Su H. et al. (2014) "Elevated snoRNA biogenesis is essential in breast cancer." Oncogene 33(11):1348-58 [PMID: 23542174]

  •  图1. nrStar™ Human snoRNA PCR Array工作流程

    适用的PCR仪:
    ABI ViiA™ 7

    ABI 7500 & ABI 7500 FAST

    ABI 7900HT

    ABI QuantStudio™ 6 Flex

    Real-Time PCR system

    ABI QuantStudio™ 7 Flex Real-Time PCR system

    ABI QuantStudio™ 12K Flex Real-Time PCR System

    Bio-Rad CFX384

    Bio-Rad iCycler & iQ Real-Time PCR Systems

    Eppendorf Realplex

    QIAGEN Rotor Gene Q100

    Roche LightCycler480

    Stratagene Mx3000

    Roche LightCycler480

  • SnoPY:
    snoRNA-LBME-db:

    Human SnoRNA Database

    C/D BOX(213)

    SNORD4A; SNORD5; SNORD6; SNORD7; SNORD8; SNORD9; SNORD10; SNORD119; SNORD121A; SNORD121B; SNORD123; SNORD124; SNORD126; SNORD127; SNORD1A; SNORD1B; SNORD1C; SNORD2; SNORD101; SNORD102; SNORD103; SNORD103B; SNORD104; SNORD105; SNORD105B; SNORD12C; SNORD13; SNORD14A; SNORD14B; SNORD15A; SNORD15B; SNORD16; SNORD18A; SNORD18B; SNORD18C; SNORD20; SNORD21; SNORD22; SNORD24; SNORD25; SNORD26; SNORD27; SNORD28; SNORD3; SNORD30; SNORD31; SNORD32A; SNORD32B; SNORD33; SNORD34; SNORD35A; SNORD35B; SNORD36A; SNORD36B; SNORD36C; SNORD37; SNORD38A; SNORD38B; SNORD41; SNORD42A; SNORD42B; SNORD43; SNORD4B; U97; SNORD96B; SNORD96A; SNORD95; SNORD94; SNORD86; SNORD84; SNORD83B; SNORD83A; SNORD117; SNORD82; SNORD81; SNORD80; SNORD118; SNORD78; SNORD77; SNORD76; SNORD75; SNORD73a; SNORD63; SNORD62B; SNORD61; SNORD60; SNORD59B; SNORD59A; SNORD58B; SNORD58C; SNORD57; SNORD56; SNORD55; SNORD54; SNORD53; SNORD52; SNORD51; SNORD50; SORD50B; SNORD48; SNORD47; SNORD46; SNORD45A; SNORD45B; SNORD45C; SNORD44; SNORD12; SNORD12B; SNORD112; SNORD113-1; SNORD114-1; SNORD17; SNORD19; SNORD19B; SNORD23; SNORD65; SNORD66; SNORD67; SNORD88A/B/C; SNORD68; SNORD69; SNORD70; SNORD71; SNORD72; SNORD85; SNORD87; SNORD90; SNORD91A; SNORD91B; SNORD92; SNORD93; SNORD98; SNORD99; SNORD100; SNORD109A/B; SNORD115-1; SNORD110; SNORD111; SNORD111B; SNORD116-1; SNORD11; SNORD11B; SNORD12; SNORD12B; SNORD113-2; SNORD113-4;SNORD113-5; SNORD113-6; SNORD113-7; SNORD113-8; SNORD113-9/ SNORD113-3; SNORD114-10/18; SNORD114-11; SNORD114-12/14; SNORD114-13; SNORD114-15/3/5/21/23/25/26; SNORD114-17/4; SNORD114-19; SNORD114-2; SNORD114-20/21/22/28; SNORD114-24; SNORD114-29/30; SNORD114-31; SNORD114-6/9; SNORD114-7; SNORD115-11; SNORD115-12; SNORD115-17/18/23; SNORD115-27/29/30; SNORD115-28; SNORD115-36; SNORD115-37; SNORD115-48; SNORD116-11; SNORD116-12/16/17/18/21/22/24; SNORD116-13/14/15/20; SNORD116-19; SNORD116-23; SNORD116-25/26; SNORD116-27/29/30; SNORD116-28; SNORD116-5; SNORD116-6; SNORD116-9; SNORD79; SNORD58A; SNORD49A; SNORD49B; SNORD45BL2; SNORD42BL1; SNORD3@L39; SNORD3@L19; SNORD118L14; SNORD3L3; SNORD118L11; SNORD68L1; SNORD118L9; SNORD3@L37; SNORD74L5; SNORD45BL1; SNORD65L2; SNORD23; SNORD38BL3; SNORD44L1; SNORD74L4; SNORD45BL3; SNORD56L7; SNORD77L3; SNORD41L1; SNORA25L14; SNORD75L2; SNORD114-15L1; U3.41-201; SNORD53L1; SNORD55L1; SNORD62BL1; SNORD51L1 

    H/ACA BOX(122)
    SNORA10; SNORA13; SNORA14A; SNORA14B; SNORA15; SNORA16; SNORA17; SNORA18; SNORA19; SNORA21; SNORA22; SNORA23; SNORA24; SNORA25; SNORA28; SNORA3; SNORA2B; SNORA45; SNORA30; SNORA31; SNORA33; SNORA34; SNORA36; SNORA36B; SNORA37; SNORA38; SNORA39; SNORA4; SNORA41; SNORA42; SNORA43; SNORA44; SNORA46; SNORA48; SNORA49; SNORA5A; SNORA50; SNORA51; SNORA52; SNORA53; SNORA54; SNORA55; SNORA56; SNORA58; SNORA59; SNORA5B; SNORA5C; SNORA6; SNORA60; SNORA61; SNORA77; SNORA80; SNORA80B; SNORA7A; SNORA8; SNORA9; SNORA62; SNORA63; SNORA47; SNORA35; SNORA26; SNORA11B; SNORA11D; SNORA11E; SNORA36C; SNORA38B; SNORA84; SNORA11; SNORA12; SNORA73A; SNORA73B; SNORA74A; SNORA74B; SNORA64; SNORA65; SNORA66; SNORA67; SNORA70; SNORA70B; SNORA70C; SNORA70D; SNORA70E; SNORA70F; SNORA71A; SNORA71B; SNORA72; SNORA99; SNORA71D; SNORA20; SNORA27; SNORA29; SNORA32; SNORA40; SNORA78; HBI-61; SNORA11C; SNORA68; SNORA69; SNORA16B; SNORA1; SNORA72L7; SNORA64L2; SNORA18L1; SNORA62L2; AL137790.4; SNORA11DL1; SNORA51L11; SNORA10L1; SNORA12L2; SNORA11EL1; SNORA70BL6; SNORA20L1; SNORA63L9; SNORA18L2; SNORA20L4; SNORA11BL2; SNORA67L1; SNORA32L2; SNORA2AL1; SNORA43L2; SNORA12L1; SNORA62L4

    Cajal body-specific scaRNAs(24)
    SCARNA18; HTR; HBII-382; SCARNA13; SCARNA8; SCARNA17; SCARNA7; SCARNA12; SCARNA6; SCARNA5; SCARNA10; SCARNA14; mgU2-25/61; SCARNA9; mgU12-22/U4-8; HBI-100; ACA68; ACA66; SCARNA11; SCARNA16; SCARNA1; SCARNA4; SCARNA23; SCARNA22

    Mouse SnoRNA Database

    C/D BOX(134)

    300055_SNORD116; 300078_SNORD49; 300079_SNORD49; 300080_SNORD65; 300108_SNORD38; 300110_SNORD50; 300116_SNORD115; 300118_SNORD115; 300144_SNORD115; 300189_SNORD113; 300194_SNORD93; 300222_SNORD115; 300273_SNORD113; 300274_SNORD113; 300275_SNORD113; 300277_SNORD113; 300278_SNORD113; 300308_SNORD58; 300357_SNORD60; 300375_SNORD12; 300441_SNORD115; 300489_SNORD86; 300524_SNORD86; 300528_SNORD113; 300529_SNORD113; 300530_SNORD113; 300531_SNORD113; 300532_SNORD113; 300537_SNORD64; 300543_SNORD89; 300559_SNORD30; 300561_SNORD113; 300562_SNORD14; 300613_SNORD99; 300615_SNORD52; 300616_SNORD48; 300617_SNORD52; 300638_SNORD78; 300639_SNORD79; 300641_SNORD47; 300642_SNORD81; 300645_SNORD115; 300647_SNORD115; 300662_SNORD115; 300674_SNORD116; 300677_SNORD70; 300706_SNORD81; 300709_SNORD25; 300710_SNORD26; 300711_SNORD27; 300712_SNORD28; 300713_SNORD29; 300714_SNORD30; 300715_SNORD31; 300716_SNORD22; 300721_SNORD87; 300732_SNORD63; 300734_SNORD58; 300739_SNORD94; 300746_SNORD23;300751_SNORD88; 300752_SNORD35; 300753_SNORD35; 300754_SNORD34; 300756_SNORD32; 300759_SNORD15; 300760_SNORD15; 300771_SNORD21; 300778_SNORD102; 300782_SNORD70; 300784_SNORD51; 300789_SNORD20; 300790_SNORD82; 300795_SNORD74; 300796_SNORD75; 300797_SNORD24; 300808_SNORD41; 300812_SNORD71; 300813_SNORD111; 300814_SNORD111; 300818_SNORD69; 300819_SNORD19; 300820_SNORD19; 300832_SNORD61; 300843_SNORD100; 300844_SNORD101; 300845_SNORD98; 300846_SNORD37; 300851_SNORD59; 300852_SNORD72; 300858_SNORD43; 300873_SNORD66; 300886_SNORD105; 300887_SNORD14; 300888_SNORD14; 300889_SNORD14; 300890_SNORD18; 300891_SNORD16; 300892_SNORD18; 300893_SNORD16; 300894_SNORD18; 300903_SNORD73; 300904_SNORD73; 300907_SNORD62; 300909_SNORD45; 300910_SNORD45; 300916_SNORD83; 300917_SNORD83; 300918_SNORD92; 300919_SNORD53; 300920_SNORD53; 300930_SNORD95; 300931_SNORD96; 300936_SNORD91; 300937_SNORD42; 300939_SNORD42; 300954_SNORD38; 300955_SNORD38; 300956_SNORD46; 300957_SNORD55; 300961_SNORD85; 300962_SNORD103; 300966_SNORD24; 300967_SNORD36; 300968_SNORD36; 300974_SNORD90; 300976_SNORD67; 300980_SNORD110; 300982_SNORD56; 300983_SNORD57; 300985_SNORD17; 300988_SNORD12; 300989_SNORD12; 300990_SNORD12


    H/ACA BOX(221)
    300000_SNORA17; 300001_SNORA65; 300003_SNORA17; 300004_SNORA73; 300010_SNORA17; 300011_SNORA17; 300013_SNORA75; 300016_SNORA51; 300019_SNORA25; 300020_SNORA43; 300021_SNORA17; 300023_SNORA2; 300025_SNORA44; 300031_SNORA17; 300033_SNORA76; 300037_SNORA17; 300038_SNORA17; 300040_SNORA17; 300044_SNORA2; 300045_SNORA44; 300049_SNORA70; 300074_SNORA63; 300075_SNORA25; 300089_SNORA58; 300092_SNORA30; 300097_SNORA8; 300098_SNORA1; 300100_SNORA32; 300102_SNORA17; 300104_SNORA73; 300111_SNORA17; 300112_SNORA32; 300113_SNORA17; 300115_SNORA70; 300167_SNORA33; 300175_SNORA73; 300177_SNORA17; 300195_SNORA17; 300196_SNORA17; 300265_SNORA17; 300266_SNORA70; 300271_SNORA70; 300279_SNORA71; 300284_SNORA17; 300285_SNORA17; 300288_SNORA70; 300289_SNORA17; 300295_SNORA71; 300304_SNORA9; 300311_SNORA61; 300312_SNORA17; 300313_SNORA17; 300315_SNORA67; 300319_SNORA32; 300320_SNORA70; 300322_SNORA38; 300327_SNORA17; 300330_SNORA28; 300343_SNORA71; 300354_SNORA46; 300358_SNORA43; 300367_SNORA65; 300368_SNORA17; 300385_SNORA17; 300391_SNORA31; 300393_SNORA17; 300398_SNORA71; 300400_SNORA46; 300401_SNORA67; 300406_SNORA17; 300410_SNORA57; 300411_SNORA67; 300413_SNORA68; 300414_SNORA17; 300417_SNORA71; 300418_SNORA71; 300421_SNORA4; 300428_SNORA55; 300430_SNORA66; 300432_SNORA17; 300434_SNORA70; 300447_SNORA17; 300451_SNORA13; 300456_SNORA2; 300458_SNORA17; 300461_SNORA2; 300464_SNORA17; 300467_SNORA42; 300468_SNORA3; 300477_SNORA24; 300479_SNORA25; 300480_SNORA17; 300486_SNORA51; 300490_SNORA55; 300491_SNORA17;300496_SNORA17; 300498_SNORA44; 300500_SNORA24; 300501_SNORA44; 300512_SNORA17; 300515_SNORA17; 300526_SNORA79; 300533_SNORA71; 300534_SNORA61; 300535_SNORA7; 300548_SNORA17; 300550_SNORA74; 300555_SNORA63; 300564_SNORA73; 300586_SNORA73; 300590_SNORA17; 300591_SNORA17; 300595_SNORA70; 300608_SNORA17; 300610_SNORA16; 300622_SNORA70; 300623_SNORA2; 300624_SNORA38; 300626_SNORA7; 300629_SNORA55; 300632_SNORA17; 300635_SNORA73; 300637_SNORA17; 300643_SNORA63; 300668_SNORA42; 300670_SNORA51; 300673_SNORA70; 300675_SNORA20; 300693_SNORA24; 300694_SNORA62; 300697_SNORA17; 300703_SNORA71; 300704_SNORA26; 300707_SNORA61; 300718_SNORA17; 300720_SNORA38; 300724_SNORA44; 300730_SNORA17; 300731_SNORA70; 300738_SNORA5; 300740_SNORA73; 300741_SNORA36; 300742_SNORA63; 300743_SNORA7; 300745_SNORA70; 300747_SNORA40; 300757_SNORA70; 300758_SNORA32; 300763_SNORA13; 300764_SNORA23; 300765_SNORA24; 300766_SNORA17; 300768_SNORA52; 300769_SNORA54; 300775_SNORA49; 300777_SNORA22; 300779_SNORA27; 300781_SNORA33; 300785_SNORA41; 300787_SNORA26; 300788_SNORA75; 300793_SNORA61; 300798_SNORA17; 300800_SNORA19;300801_SNORA42; 300802_SNORA12; 300803_SNORA32; 300805_SNORA3; 300807_SNORA68; 300809_SNORA46; 300810_SNORA50; 300817_SNORA14; 300821_SNORA74; 300823_SNORA26; 300824_SNORA36; 300825_SNORA31; 300826_SNORA5; 300828_SNORA36; 300829_SNORA69; 300831_SNORA17; 300834_SNORA72; 300835_SNORA35; 300836_SNORA11; 300839_SNORA70; 300847_SNORA53; 300848_SNORA79; 300853_SNORA17; 300854_SNORA72; 300856_SNORA11; 300859_SNORA2; 300861_SNORA73; 300862_SNORA55; 300863_SNORA24; 300870_SNORA17; 300872_SNORA77; 300874_SNORA63; 300875_SNORA63; 300877_SNORA17; 300882_SNORA2; 300883_SNORA24; 300897_SNORA61; 300898_SNORA62; 300899_SNORA73; 300900_SNORA62; 300901_SNORA69; 300905_SNORA58; 300908_SNORA5; 300912_SNORA29; 300913_SNORA20; 300914_SNORA64; 300923_SNORA21; 300926_SNORA28; 300927_SNORA5; 300929_SNORA24; 300933_SNORA29; 300949_SNORA17; 300958_SNORA18; 300965_SNORA32; 300977_SNORA11; 300979_SNORA25; 300986_SNORA71

    Other snoRNAs(15)
    300032_SNORND104; 300099_snoZ40;300317_snoZ30; 300640_snosnR60_Z15; 300755_snoZ195; 300792_snoU6-53; 300816_snoMBII-202; 300822_snoU6-53; 300857_snoU83B; 300871_snoU2-30; 300884_snoU2-30; 300938_snoZ17; 300947_snR38-2; 300948_snR38-3


  • Aksomics使用Arraystar的芯片为客户提供服务,以下为相关资料:


    产品说明书:  adobe_pdf_small Manual_nrStar™_Human_snoRNA_PCR_Array_v1.03

                                adobe_pdf_small Manual_nrStar™ Mouse snoRNA PCR Array_v1.0

    分析工具: excelAnalysis Tool for nrStar™ Human snoRNA PCR Array

                           excelAnalysis_Tool_for_nrStar™_Mouse_snoRNA_PCR_Array

    分析工具说明书:adobe_pdf_small Instructions of snoRNA qPCR data analysis tool